OZONE THERAPY RESEARCH
CANCER
Effect of a parenteral ozone-oxygen mixture on the concentration of immunoglobulins (IgA, IgG, IgM), of vitamin A and lysozyme activity in patients with cervical cancer.
Geburtshilfe Frauenheilkd. 1987 Dec;47(12):343-5.
Enzelsberger H1, Metka M, Salzer H.
Abstract
In the literature you can find several therapeutic possibilities in the treatment of carcinoma by additive ozone therapy. We investigated the consequences of ozone therapy for the immunological status, lysozyme and vitamin A. 21 women with progressive cervical cancer (Stage III, IV) got besides the conventional irradiation therapy also an additive ozone therapy. After irradiation and ozone therapy a small decrease in IgG, IgA and IgM can be seen. A statistical significance could not be evaluated. There was no difference to the control group in lysozyme and vitamin A.
Ozone Therapy in the Management of Persistent Radiation-Induced Rectal Bleeding in Prostate Cancer Patients.
Evid Based Complement Alternat Med. 2015;2015:480369. doi: 10.1155/2015/480369. Epub 2015 Aug 18.
Clavo B1, Santana-Rodriguez N2, Llontop P3, Gutierrez D4, Ceballos D5, Méndez C3, Rovira G6, Suarez G4, Rey-Baltar D4, Garcia-Cabrera L7, Martínez-Sánchez G8, Fiuza D3.
Abstract
Introduction. Persistent radiation-induced proctitis and rectal bleeding are debilitating complications with limited therapeutic options. We present our experience with ozone therapy in the management of such refractory rectal bleeding. Methods. Patients (n = 12) previously irradiated for prostatecancer with persistent or severe rectal bleeding without response to conventional treatment were enrolled to receive ozone therapy via rectal insufflations and/or topical application of ozonized-oil. Ten (83%) patients had Grade 3 or Grade 4 toxicity. Median follow-up after ozone therapy was 104 months (range: 52-119). Results. Following ozone therapy, the median grade of toxicity improved from 3 to 1 (p < 0.001) and the number of endoscopy treatments from 37 to 4 (p = 0.032). Hemoglobin levels changed from 11.1 (7-14) g/dL to 13 (10-15) g/dL, before and after ozonetherapy, respectively (p = 0.008). Ozone therapy was well tolerated and no adverse effects were noted, except soft and temporary flatulence for some hours after each session. Conclusions.Ozone therapy was effective in radiation-induced rectal bleeding in prostate cancer patients without serious adverse events. It proved useful in the management of rectal bleeding and merits further evaluation.
The use of ozone in the prevention of osteoradionecrosis of the jaw.
Saudi Med J. 2014 Oct;35(10):1260-3.
Batinjan G1, Filipovic Zore I, Vuletic M, Rupic I.
Abstract
Radiation therapy of the head and neck area has resulted in numerous and undesired effects on the oral cavity tissue. The most frequent and most dangerous complications of radiotherapy of the head and neck area is osteoradionecrosis (ORN). The purpose of this study is to demonstrate the possibility of using ozone in the prevention of ORN of the jaw, and show the recent therapeutic protocols for treatment in patients receiving radiationtherapy for the head and neck. We used an ElektroMagneTron device during the therapy. The patient underwent postoperative follow-up examination when bio-oxygenation therapy and clinical evaluation of wound healing were performed. Wound healing evaluation showed no complications. Ozonetherapy is given as an option in the therapeutic protocol for the prevention and treatment of ORN.
Adjuvant aqueous ozone in the treatment of bisphosphonate induced necrosis of the jaws: report of two cases and long-term follow-up.
Minerva Stomatol. 2014 Jan-Feb;63(1-2):35-41.
Brozoski MA1, Lemos CA, Da Graça Naclério-Homem M, Deboni MC.
Abstract
Bisphosphonate induced necrosis of the jaws (BONJ) does not have a unique protocol of treatment and many therapeutic approaches have been arising in oral medicine with debatable results. A male and a female attended the University Oral Surgery Clinic presenting oral bone lesions induced by intravenous and oral bisphosphonates respectively as complications of dental extraction. Treatment included daily mouthwashes and weekly intra oral irrigations with 4 mg/L of aqueous-ozone, antibiotic therapy and sequential superficial debridment for sequestrectomies. Long-standing follow-ups showed complete mucosa covering of exposed bone area and resolution of purulent secretion. Antibacterial and antifungal properties of aqueousozone may have played important roles in the treatment. The outcome measured intra oral examination and panoramic radiographs of the affected bone. The application of aqueous ozone daily mouthwashes and weekly professional irrigation were safe; free from adverse effects, easily of handling and worked as an important adjuvant therapeutic strategy for the treatment of BONJ.
Treatment of osteonecrosis of the jaw (ONJ) by medical ozone gas insufflation. A case report.
Tumori. 2012 May-Jun;98(3):72e-75e. doi: 10.1700/1125.12414.
Ripamonti CI1, Maniezzo M, Pessi MA, Boldini S.
Abstract
Osteonecrosis of the jaw (ONJ) is an adverse event reported in patients receiving cancertreatment, especially bisphosphonates and denosumab. A patient with multiple myeloma who underwent up to 22 intravenous zoledronic acid infusions without previous dental examination developed a devastating ONJ lesion in the right horizontal mandibular ramus. He was treated with local ozone gas applications every third day by means of a special bell to avoid O(3) diffusion. Azithromycin 500 mg/day was administered for 10 days prior to the O(3) applications. O(3) therapyresulted in sequestration of the necrotic bone after a median of 15 applications, following which surgery was necessary to remove it. Interestingly, removal was possible without the resection of the healthy mandible edge because of the presence of the bone sequestrum. Medical O(3) gas administration was effective and safe in a patient treated with bisphosphonates who developed a >2.5 cm ONJ lesion. Future research is needed to demonstrate the efficacy and tolerability of suchtreatment in a larger number of patients.
Efficacy and safety of medical ozone (O(3)) delivered in oil suspension applications for the treatment of osteonecrosis of the jaw in patients with bone metastases treated with bisphosphonates: Preliminary results of a phase I-II study.
Oral Oncol. 2011 Mar;47(3):185-90. doi: 10.1016/j.oraloncology.2011.01.002.
Ripamonti CI1, Cislaghi E, Mariani L, Maniezzo M.
Abstract
Osteonecrosis of the jaw (ONJ) is an adverse event that has been reported in patients receiving cancer treatment regimens, including bevacizumab, bisphosphonates, and denosumab. We performed a preliminary open label, prospective phase I-II study in patients treated with bisphosphonate to evaluate the treatment effect and tolerability of medical ozone (O(3)) delivered in an oil suspension on BONJ lesions ≤2.5cm. Ten consecutive patients with BONJ lesions not responsive to conservative treatment were pre-treated with 10days of antibiotics to reduce purulent secretions on the gum. The exposed bone lesion and osteomucosal edge was cleaned with an ultrasonic scaler. The BONJ lesion was treated with 10 local applications of medical O(3) delivered in an oil suspension for 10min. In all patients, mucosal lesions resolved with complete reconstitution of oral and jaw tissue, with 3-10 applications. No toxicity was reported. Unexpectedly, total sequestration of the necrotic bone, with spontaneous expulsion in eight patients and new bone formation around the necrotic area in two patients was observed. No patient required surgical intervention. In two patients with pre-and post-treatment X-rays, no residual bone lesions were observed after treatment. These preliminary results show the efficacy and tolerability of O(3) delivered in an oil suspension applied directly to BONJ lesions ≤2.5cm, thus indicating that BONJ can be a manageable and potentially curable condition.
Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats.
Delgado-Roche L1, Hernández-Matos Y1, Medina EA1, Morejón DÁ1, González MR2, Martínez-Sánchez G3.
Sultan Qaboos Univ Med J. 2014 Aug;14(3):e342-8. Epub 2014 Jul 24.
OBJECTIVES:
Induced dilated cardiomyopathy is the main limitation of the anti-cancer drug doxorubicin, which causes oxidative stress and cardiomyocyte death. As ozone therapy can activate the antioxidant systems, this study aimed to investigate the therapeutic efficacy of ozone-oxidative preconditioning against doxorubicin-induced cardiotoxicity.
METHODS:
The study was carried out from September 2013 to January 2014. Sprague-Dawley rats were randomly distributed in the followingtreatment groups: Group 1 were treated with 2 mg/kg intraperitoneal (i.p.) of doxorubicin twice a week for 50 days; Group 2 were treated with 0.3 mg of ozone/oxygen mixture at 50 μg/mL of ozone per 6 mL of oxygen by rectal insufflation and then treated with doxorubicin; Group 3 were treated as Group 2 but only with the oxygen, and Group 4 were treated with oxygen first, and then with sodium chloride i.p. as the control group.
RESULTS:
The results showed that ozone therapy preserved left ventricle morphology which was accompanied by a reduction of serum pro-brain natriuretic peptide levels. The cardioprotective effects of ozone-oxidative preconditioning were associated with a significant increase (P <0.05) of antioxidant enzymes activities and a reduction of lipid and protein oxidation (P <0.05).
CONCLUSION:
Ozone-oxidative preconditioning prevents doxorubicin-induced dilated cardiomyopathy through an increase of antioxidant enzymes and a reduction of oxidised macromolecules. This establishes the background for future studies to determine if ozone therapy can be used as a complementary treatment for attenuating doxorubicin-induced cardiotoxicity in cancer patients.
Restoration of normoxia by ozone therapy may control neoplastic growth: a review and a working hypothesis.
Bocci V1, Larini A, Micheli V.
J Altern Complement Med. 2005 Apr;11(2):257-65.
Abstract
In contrast to normal tissues, tumors thrive in hypoxic environments. This appears to be because they can metastasize and secrete angiopoietins for enhancing neoangiogenesis and further tumor spread. Thus, during chronic ischemia, normal tissues tend to die, while neoplasms tend to grow. During the past two decades, it has been shown in arteriopathic patients that ozonated autohemotherapy is therapeutically useful because it increases oxygen delivery in hypoxic tissues, leading to normoxia. Although several oxygenation approaches have been tested, none is able to restore normoxia permanently in patients with cancer. We postulate that a prolonged cycle of ozonated autohemotherapy may correct tumor hypoxia, lead to less aggressive tumor behavior, and represent a valid adjuvant during or after chemo- or radiotherapy. Moreover, it may re-equilibrate the chronic oxidative stress and reduce fatigue.
Ozone ameliorates methotrexate-induced intestinal injury in rats.
Kesik V1, Uysal B, Kurt B, Kismet E, Koseoglu V.
Cancer Biol Ther. 2009 Sep;8(17):1623-8. Epub 2009 Sep 6.
Abstract
Methotrexate (Mtx) is an effective chemotherapeutic agent used in various cancer treatments. Gastrointestinal toxicity is the drug's major limiting factor, arising mainly from oxidative damage. It has been proposed that ozone (O(3)) is an activator of antioxidant enzymes. Thus, this study was designed to investigate the efficacy of ozone therapy in the prevention of Mtx-induced intestinal injury in rats. Twenty rats were allocated into three groups: sham, Mtx alone (untreated) and Mtx + O(3) (treated with ozone). Ozone was administered at a dose of 0.72 mg/kg daily via an intraperitoneal route for 15 d. On d 16, Mtx was applied via an intraperitoneal injection at a dose of 6 mg/kg for 5 d. All rats were sacrificed at d 21. Efficacy of the treatment was assessed by measuring the histopathologic injury score (HIS), and biochemically by determining tissue superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in ileum, liver and kidney homogenates. Although two rats (25%) died in the untreated group, all rats in the sham and treatment groups survived the study. The HIS, antioxidant enzyme and MDA levels of the ileal tissue were significantly lower in the ozone treated group than the untreated group (p < 0.05). Although the antioxidant enzyme and MDA levels of liver and kidney were significantly lower in the ozone treated group (p < 0.05), there was no significant change in histopathology (p > 0.05). Thus,ozone preconditioning shows a preventative effect in the ileum by decreasing tissue damage and increasing antioxidant enzyme activity in an experimental model of Mtx-induced intestinal injury.
Treatment with ozone/oxygen-pneumoperitoneum results in complete remission of rabbit squamous cell carcinomas.
Int J Cancer. 2008 May 15;122(10):2360-7. doi: 10.1002/ijc.23382.
Schulz S1, Häussler U, Mandic R, Heverhagen JT, Neubauer A, Dünne AA, Werner JA, Weihe E, Bette M.
Abstract
Head and neck squamous cell carcinomas (HNSCC) represent a group of metastasizing tumors with a high mortality rate in man and animals. Since the biomolecule ozone was found to inhibit growth of various carcinoma cells in vitro we here applied the highly aggressive and lethal VX2 carcinoma HNSCC tumor model of the New Zealand White rabbit to test whether ozone exerts antitumorous effects in vivo. Therapeutic insufflation of medicalozone/oxygen (O(3)/O(2)) gas mixture into the peritoneum (O(3)/O(2)-pneumoperitoneum) at an advanced stage of tumor disease led to a survival rate of 7/14 rabbits. Six of the seven surviving rabbits presented full tumor regression and the absence of local or distant lung metastases. Insufflation of pure oxygen (O(2)) resulted in a survival rate of 3/13 animals accompanied by full tumor remission in 2 of the 3 surviving animals. Of the 14 sham-treated animals only 1 had spontaneous tumor remission and survived. No adverse effects or changes in standard blood parameters were observed after repeated intraperitoneal insufflations of the O(3)/O(2) or O(2) gas. Animals with O(3)/O(2)-induced tumor eradication developed tolerance against reimplantation of the VX2 tumor. This could be reversed by immune suppression with a combination of dexamethasone and cyclosporin A suggesting an antitumorous effect of O(3)/O(2)-mediated activation of the body's own immunosurveillance. Although the exact mechanisms of action are still unclear the present data point to O(3)/O(2)-pneumoperitoneum as a promising new strategy in anticancer therapy.
Ozone Therapy in Cancer Treatment: State of the Art
Ozone: Science & Engineering. The Journal of the International Ozone Association. 2011 May 23. 398-404. DOI: 10.1080/01919512.2012.718700.
Silvia Menéndeza*, Janet Ceperob & Luis Borregoc
Erhlich Ascitic Tumor and Sarcoma 37 were implanted in mice and afterward the animals were treated with ozone (rectally). A significant decrease in the number of metastasis was obtained. In another study, ozone was applied intraperitoneally, before Lewis' lung carcinoma inoculation. A delayed effect in the tumor development kinetics and in the increase rate of tumor volume in the ozone groups was observed. With regard to the clinical trial, patients with prostatic cancer were treated with cobalt-60 therapy and ozone (rectally), decreasing the presence of side effects (due to radiation treatment) and the prostatic specific antigen figures. However, further investigations are necessary to be performed, in order to be considered the ozone therapy as complementary therapy for cancer.
OZONE IN GASTROENTEROLOGY
Efficacy of hyperbaric oxygen therapy and medical ozone therapy in experimental acute necrotizing pancreatitis.
Pancreas. 2010 Jan;39(1):9-15. doi: 10.1097/MPA.0b013e3181bb5ae3.
Uysal B1, Yasar M, Ersoz N, Coskun O, Kilic A, Cayc T, Kurt B, Oter S, Korkmaz A, Guven A.
Abstract
OBJECTIVES:
Our aims were to evaluate the efficacy of ozone therapy (OT) in an experimental rat model of acute necrotizing pancreatitis (ANP) and to compare its effects with hyperbaric oxygen (HBO) therapy in this entity.
METHODS:
Forty Sprague-Dawley rats were divided into sham-operated, ANP, ANP + HBO, and ANP + OT groups. Acute necrotizing pancreatitis was induced by infusing 1-mL/kg 3% sodium taurocholate into the common biliopancreatic duct. Hyperbaric oxygen was administered twice daily at a 2.8-atm pressure for 90 minutes. Ozone therapy was set as daily intraperitoneal injections of 0.7-mg/kg ozone/oxygen gas mixture. Hyperbaric oxygen and OT were continued for 3 days after the induction of ANP. The surviving animals were killed at the fourth day, and their pancreases were harvested for biochemical, microbiological, and histopathologic analyses.
RESULTS:
Serum amylase/lipase and neopterin levels and tissue oxidative stress parameters were similar to sham's values in both the ANP + HBO and the ANP + OT groups. Histopathologic injury scores were significantly lower in the treatments groups than in the ANP group. When compared with the ANP group, the number of infected rats was significantly lesser in the ANP + HBO and the ANP + OT groups.
CONCLUSIONS:
Hyperbaric oxygen and OT reduce the severity and the mortality in the experimental rat model of ANP, and a greater benefit was received for OT comparing with HBO.
Ozone Therapy in Patients with Viral Hepatitis C: Ten Years' Experience
DOI:10.1080/01919512.2012.720161
M.N. Mawsoufa*, T.T. Tanboulib & R. Viebahn-Hänslerc
pages 451-458
Abstract
Hepatitis C is a medical problem in Egypt. The usual line of treatment is very expensive, with major side effects and low efficacy especially in genotype 4, which is common in Egypt. Several studies were performed between 1999 and 2008 to evaluate the role of ozone therapy in HCV (Hepatitis C). The first study included 60 genotype 4 Hepatitis C patients, who received combined ozone treatment with major autohemotherapy three times per week for 8 weeks, followed by twice per week for 16 weeks. It was found that, following 8 weeks of ozone therapy, the viral load decreased in 91.67% of the cases attaining negative PCR in 20%. Following 24 weeks of ozone therapy, there was a further decrease in viral load reaching 95% of the cases, with a negative PCR level in 36.67%. After 8 weeks of ozone therapy, the abnormal enzyme levels were back to normal in 21.67% of the cases for the SGPT enzyme, and were back to normal in 20% for the SGOT enzyme. A second study included 50 genotype 4 Hepatitis C patients. The number of visits was three times per week for 12 weeks followed by twice per week for 12 weeks. The general condition improved in 94% of the cases. There was a decrease in quantitative PCR in 71.8% of the cases that reached negative PCR in 24% after 8 weeks' treatment. The number of negative PCR cases for HCV virus increased to cover 36% of the cases after 24 weeks' treatment. There was a statistically significant improvement as regards the parameters of SGOT, SGPT, albumin, bilirubin and prothrombin after 8 weeks from the start of the study. A third study was carried out on 30 HCV patients, yielding results similar to the previous two.
The efficacy of ozone therapy in experimental caustic esophageal burn.
J Pediatr Surg. 2008 Sep;43(9):1679-84. doi: 10.1016/j.jpedsurg.2008.01.064.
Guven A1, Gundogdu G, Sadir S, Topal T, Erdogan E, Korkmaz A, Surer I, Ozturk H.
Abstract
INTRODUCTION:
Ozone has been proposed as an antioxidant enzyme activator, immunomodulator and cellular metabolic activator. This study was designed to investigate the efficacy of ozone therapy in the prevention ofesophageal damage and stricture formation developed after esophageal caustic injuries in the rat.
MATERIALS AND METHODS:
Forty-five rats were allocated into three groups; sham-operated, un-treatment and treatment groups. Caustic esophageal burn was created by instilling 15% NaOH in the distal esophagus. The rats were left untreated or treated with 1 mg/kg/day ozone intraperitoneally. All rats were sacrificed at 28 days. Efficacyof the treatment was assessed by measuring the stenosis index (SI) and histopathologic damage score, and biochemically by determining tissue hydroxyproline content (HP), superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) and protein carbonyl content (PCC) in esophageal homogenates.
RESULTS:
Whereas seven (47%) rats died in the un-treatment group, all rats in the sham-operated and the treatment group survived during the study. SI, the histopathologic damage score, was significantly lower in theozone-therapy group than the un-treatment group. HP levels were significantly higher in the un-treatment group than the group treated with ozone. Caustic esophageal burn increased MDA and PCC levels and also decreased SOD and GPx enzyme activities. In contrast, ozone therapy decreased the elevated MDA and PCC levels and also increased the reduced SOD and GPx enzyme activities.
CONCLUSION:
Ozone has a preventive effect in the development of fibrosis by decreasing tissue damage and increasing the antioxidant enzyme activity in an experimental model of corrosive esophageal injury.
Preliminary Results of Ozone Therapy as a Possible Treatment for Patients with Chronic Hepatitis C
To cite this article:
Saad Zaky, Sherif Ebrahiem Kamel, Magda Shahata Hassan, Nadia Abdel Sallam, Mohamad Ahmad Shahata, Shaaban Redwan Helal, and Heba Mahmoud. The Journal of Alternative and Complementary Medicine. March 2011, 17(3): 259-263. doi:10.1089/acm.2010.0016.
Published in Volume: 17 Issue 3: March 20, 2011
ABSTRACT
Background: Medical ozone is more bactericidal, fungicidal, and virucidal than any other natural substance. Some studies proved that ozone infused into donated blood samples can kill viruses 100% of the time. Ozone, because of its special biologic properties, has theoretical and practical attributes to make it a potent hepatitis C virus (HCV) inactivator, which suggests an important role in the therapy for hepatitis C.
Aim: The study aim is to evaluate the role of ozone therapy in decreasing HCV ribonucleic acid (HCV RNA) load and its effect on the liver enzymes among patients with chronic hepatitis C.
Methods: This study included 52 patients with chronic hepatitis C (positive polymerase chain reaction [PCR] for HCV RNA and raised serum alanine transaminase [ALT] for more than 6 months). All patients were subjected to meticulous history taking and clinical examination. Complete blood count, liver function tests, and abdominal ultrasonography were requested for all patients. The ozone group included 40 patients who received major autohemotherapy, minor autohemotherapy, and rectal ozone insufflation. The other 12 patients (conventional group) received silymarin and/or multivitamins.
Results: There were significant improvements of most of the presenting symptoms of the patients in the ozone group in comparison to the conventional group. ALT and aspartate transaminase (AST) levels normalized in 57.5% and 60% in the ozone group, respectively, in comparison to 16.7% and 8% in the conventional group, respectively. Polymerase chain reaction (PCR) for HCV RNA was negative among 25% and 44.4% after 30 and 60 sessions of ozone therapy, respectively, in comparison to 8% among the conventional group.
Conclusions: Ozone therapy significantly improves the clinical symptoms associated with chronic hepatitis C and is associated with normalized ALT and AST levels among a significant number of patients. Ozone therapy is associated with disappearance of HCV RNA from the serum (-ve PCR for HCV RNA) in 25%–45% of patients with chronic hepatitis C.
Effect of medical ozone therapy on renal blood flow and renal function of patients with chronic severe hepatitis.
Gu XB1, Yang XJ, Zhu HY, Xu YQ, Liu XY.
Chin Med J (Engl). 2010 Sep;123(18):2510-3.
Abstract
BACKGROUND:
Medical ozone therapy system was reported to have certain effects on the treatment of severe hepatitis, but its mechanism is not very clear. One of the causes of death of severe hepatitis is complication of renal damage or hepatorenal syndrome. The present study aimed to observe effects of medical ozone therapy system on plasma renin activity (PRA), angiotensin II (AII), aldosterone (ALD), renal blood flow and renal function of patients with chronic severe hepatitis and explore mechanisms of medical ozone therapy in the treatment of severe hepatitis.
METHODS:
Eighty-five cases with chronic severe hepatitis were randomly divided into ozone therapy group (43 cases) and control group (42 cases). The patients in the ozone therapy group were treated with basic treatments plus ozone therapy system. Basic autohemotherapy was used. One hundred milliliter venous blood was drawn from each patient, and was mixed with 100 ml (35 µg/ml) medical ozone and then was returned the blood to the patient intravenously, once every other day for 20 days. Only the basic treatments were given to the control group. PRA, AII, ALD, renal blood flow and damage to renal function of the two groups before treatment and 20 days after treatment were compared. Survival rates were also compared.
RESULTS:
Twenty days after the treatment, in ozone therapy group, PRA was (1.31 ± 0.12) ng·ml⁻¹·h⁻¹, AII (111.25 ± 17.35) pg/ml, ALD (251.31 ± 22.60) pg/ml, which decreased significantly compared with those before treatment (PRA (2.23 ± 0.13) ng·ml⁻¹·h⁻¹, AII (155.18 ± 19.13) pg/ml, ALD (405.31 ± 29.88) pg/ml, t = 4.67 - 14.23, P < 0.01), also lower than those of control group 20 days after the treatment (PRA (2.02 ± 0.11) ng·ml⁻¹·h⁻¹, AII (162.21 ± 15.32) pg/ml, ALD (401.20 ± 35.02) pg/ml, t = 4.97 - 15.61, P < 0.01); renal blood flow was (175.15 ± 28.20) ml/min, which increased compared with that before the treatment ((125.68 ± 21.25) ml/min) and was higher than that of control group 20 days after the treatment ((128.59 ± 23.15) ml/min, t = 4.78, 4.61, P < 0.01). Renal damage occurred in 2 cases (5%) in ozone therapy group, less than that in control group (9 cases, 21%) (χ² = 5.295, P < 0.05). Thirty-three cases (77%) in ozone therapy group vs. 16 cases (38%) in control group survived (χ² = 12.993, P < 0.01).
CONCLUSIONS:
Basic treatment plus medical ozone therapy for patients with chronic severe hepatitis could decrease PRA, AII and ALD levels significantly increase renal blood flow, prevent renal damage to certain extent and improve survival rate of the patients.
OZONE IN OPHTHALMOLOGY
Effectiveness of the ozone therapy in dry macular degeneration associated with the age.
MEDISAN [online]. 2011, vol.15, n.6, pp. 798-807. ISSN 1029-3019. VERA VIDAL, Vivian et al.
Introduction: The origin of the dry macular degeneration associated with the age is still uncertain. Objective: To determine the effectiveness of the ozone therapy in patients with this condition. Methods: A therapeutic intervention was carried out in those affected by that disease, attended in the Retina Department of the Ophthalmology Center of "Dr. Juan Bruno Zayas Alfonso" Teaching General Hospital in Santiago de Cuba, from January 2008 to January 2009. Cases were divided into two groups: those treated with ozone and those that received Nutriforte tablets. Results: The female sex and mean age of 65 years prevailed. In the first group the visual acuity of 0,51 became 0,63 after treatment, and in the second group it was 0,47 to 0,5 with improved retinal sensibility in both cases.Conclusions: Ozone therapy was effective to treat people with macular degeneration.
Effects of oxygen-ozone therapy on age-related degenerative retinal maculopathy.
Panminerva Med. 1990 Apr-Jun;32(2):77-84. Riva Sanseverino E1, Meduri RA, Pizzino A, Prantera M, Martini E.
Abstract
The effects of oxygen-ozone therapy on 20 patients affected by age-related degenerative maculopathy have been studied. Visual acuity and eye fluorangiography were the parameters used in order to evaluate the efficacy of the therapy. Medical ozone was administered intravenously according to the technique called "ozonized major autohemoinfusion", the total amount of ozone ranging from 1,500 to 2,000 micrograms per session for a 4 months period. The results have indicated that the majority of patients showed an improvement of their ocular condition, suggesting continuation of this type of investigation on a larger group of people.
Ozone Therapy in Senile Macular Degeneration.
Soto G., Falcón J., Montesino I. and Soler L. (Cuba)
Taking into account the incidence of senile macular degeneration (SMD) and low efficacy of conventional treatment, the aim of this study is to evaluate the efficacy of ozone therapy in patients with SMD of dry type. A retrospective study was performed in 22 patients assisted at the Ophthalmologic Service of the Medical Surgical Research Center (CIMEQ). Patients were diagnosed through a complete ophthalmologic examination and fluorescent angiography. Ozone was applied by major autohemotherapy (at an ozone concentration of 40 mg/L and 100 mL) and by rectal application (at an ozone concentration of 40 mg/L and 200 mL), during 20 sessions. A high percent of patients (54.5%) treated were between 61 and 70 years. White race predominate (45.4%), as well as female sex (68.2%). The results demonstrated an improvement (with an average of 2 tenths-visions) of their visual acuity in 80% of the patients treated with ozone. Ozone therapy could be a good therapeutic choice for patients suffering of SMD of dry type.
Ozone and antioxidants in (dry) macular degeneration related to age.
AMC [online]. 2008, vol.12, n.3, pp. 0-0. ISSN 1025-0255. CHAVEZ PARDO, Iris; THAIS GONZALEZ RODRIGUEZ, Noris; AVILEZ CARMENATE, Elizabeth y CARDOSO GUILLEN, Elias.
Background: Macular degeneration is recognized like the most important cause of serious and irreversible loss of vision in developed countries.
Objective: To demonstrate the visual improvement with the use of antioxidants and ozone in (dry) macular degeneration treatment related to age in patients with visual acuity until 0.5 no in the ones that present greater deficit of it.
Method: A descriptive cross-sectional study in 50 patients with diagnosis of (dry) macular degeneration related to age was carried out, from October to December 2005, attended at «Manuel Ascunce Domenech» Provincial Hospital of Camagüey. The treatment to administer was analysed (ozone therapy by rectal tract, 20 sessions, associated to antioxidants administration), as well as the age, sex and race, the referred symptoms by the patient, the visual acuity before and after treatment, (monthly, to the 3 months) and the state of satisfaction after treatment.
Results: White race in 35 patients predominated and the female sex in 25 patients. The decrease of visual acuity, and metamorphopsia were the most frequent symptoms detected.
Conclusions: The visual acuity in our patients was recovered in the 96% of the treated cases; the state of satisfaction was good after treatment
Amblyopia by Anisometropia. A New Therapeutic Method.
A. Torrado, R. Wong, G. Soto. Medical and Surgical Research Center, Cuba.
Thirty adult patients with the diagnosis of amblyopia by anisometropia were studied. The majority of the patients had high ametropia, predominantly myopia. Ozone therapy was used, associated to the usual treatment of visual exercises and macular stimulation. The use of ozone associated to traditional methods produced a better and more effective response in the recovery of the patient visual acuity, as well as shortening the treatment.
Effects of major ozonated autohemotherapy in the treatment of dry age related macular degeneration: a randomized controlled clinical study.
Int J Ophthalmol. 2012;5(6):708-13. doi: 10.3980/j.issn.2222-3959.2012.06.11. Epub 2012 Dec 18. Borrelli E1, Diadori A, Zalaffi A, Bocci V.
AIM: To evaluate the effect of systemic ozonated major autohaemotherapy (O(3)-AHT) in patients affected by dry age related macular degeneration (AMD).
METHODS: This study was a randomized, controlled clinical study. One hundred and forty patients with the diagnosis of AMD in both eyes, with the study eye presenting dry AMD and soft drusen, were randomly assigned in a 1:1 ratio to either receive 27 major ozonated autohemotherapy treatments during 12-month period, or a standardized multi-vitamin therapy. Primary outcome was the change in best corrected visual acuity (mean logMar change) between the baseline and 6 and 12 months, end point of the study. In addition, to investigate the safety of prolonged ozonated autohaemotherapy, we measured the routine haematochemical parameters and biochemical oxidative stress values at baseline and after 12 months treatment time.
RESULTS: The mean baseline best corrected visual acuity in study eyes was 0.36 in the treatment group and 0.38 in the control group (difference not statistically significant). At the primary endpoint, 6 months post-baseline, the mean logMAR change in the treated group improved by 0.1 and the values of the control group at the same time impaired by 0.2 respect to the baseline. Four percent and twenty-five percent of eyes in the group treated with O(3)-AHT gained 1 or more lines after 6 and 12 months respectively compared to 0% in the eyes which received no treatment (P<0.05 at 12 months). None of the treated patients experienced a loss in visual acuity in their study eye at 6 and 12 months, compared to 16% and 40 % of patients in the control group who lost 2 lines or more at 6 months and 12 months respectively (P<0.05 treated vs control group)). Major ozonated autohemotherapy was shown to be safe and well- tolerated by the patients. Moreover, the haematochemical parameters showed a decrease in the Reactive Oxygen Metabolites (300±10.1 UCARR at 12 months compared to a baseline value of 380±10.4 UCARR, P<0.05) and an increase in Biological Antioxidant Potential plasma values (2100±34.8 micromoles/ C vitamin after 12 months compared to the baseline value of 1610±36.2, P<0.05) in the treated patients when compared to the control group. This data suggests that major ozonated autohaemotherapy may exert a role in reducing oxidative stress by endogenously stimulating the production of antioxidant molecules.
CONCLUSION: The results of this study suggests that major ozonated autohaemotherapy could be a safe and effective therapeutic option for high-risk patients with dry AMD, and that a series of such treatments could improve the natural course of AMD.
[Effects of ozone therapy on the functional activity of the retina in patients with involutional central chorioretinal dystrophy].
Vestn Oftalmol. 2003 Nov-Dec;119(6):18-21.Neroev VV, Zueva MV, Tsapenko IV, Khandzhian AT.
Abstract
The retinal electrogenesis was studied in central chorioretinal dystrophy (CCRD) of different stages. The general peculiarities of the ozone-therapy influence on the functional retinal activity typical of any CCRD stage were detected; they comprise: an increasing activity of neurons in the muscular region and a specificity related with the dynamic functional activity of the retinal peripheral segments peculiar of each disease stage. A sharp growth of glial index Cg (b/R12) detected in all patients is indicative of an activated metabolism of Mueller glial cells and of impaired glial-neuron relations in the retina as observed in CCRD. A decreasing Cg, observed after ozone-therapy, is an indirect confirmation of anti-hypoxic effect produced by ozone, which can be regarded as a patho-physiological mechanism of its positive influence on the dynamics of the functional retinal activity. Ozone-therapy is recommended to be implemented with the electroretinography monitoring.
[Impact of ozone therapy on the electrophysiological parameters of the retina in patients operated on for its rhegmatogenous detachment].
Vestn Oftalmol. 2007 Sep-Oct;123(5):33-6.. Neroev VV, Sarygina OI, Zueva MV, Tsapenko IV, Egorova EN.
Abstract
The impact of ozone therapy on changes in the data of maximum combined electroretinography (ERG), macular ERG (MERG), and rhythmic ERG (RERG) at 12, 32, and 40 Hz was studied in the rehabilitative period in patients operated on for rhegmatogenous retinal detachment. The integral and local glial indices K(g) and K(c) were calculated. Studies were conducted before, within 1 week and 1 and 2 months after a course of therapy. With ozone therapy, ERG and RERG depended on the specific features of changes available in the fundus of the eye before surgery. In the early rehabilitative period after surgery (circlage with subretinal fluid removal), ozone therapy normalized the glial indices, by decreasing the supernormal activity of Muller's glial cells and drastically increasing their reduced activity. The maximum effect was revealed for the function of phororeceptors (except for the eyes showing pronounced myopic dystrophic changes in the fundus). There were increases in MERG, ERG b-wave, and low-frequency RERG by 12 Hz, i.e. improved functions of the macular region and distal neurons of the retinal rod cell system. A latent period was established for the manifestation of a positive effect of ozone therapy on the functional of neurons of the internal retinal nuclear layer, as evidenced by RERG changes. Ozone therapy positively affect retinal electrogenesis in cases of total retinal detachment accompanied by a pronounced reduction in the glial index Kg before surgery. The therapy was found to have a positive impact on the functional activity of the retina of pair eyes, by developing within a one-moth latent period.
Evaluation of patients with retinitis pigmentosa receiving electric stimulation, ozonated blood, and ocular surgery in Cuba.
Arch Ophthalmol. 1996 May;114(5):560-3.Berson EL1, Remulla JF, Rosner B, Sandberg MA, Weigel-DiFranco C.
Abstract
OBJECTIVE: To evaluate the effect of intervention with electric stimulation, autotransfused ozonated blood, and ocular surgery, performed in Cuba, on the course of the common forms of retinitis pigmentosa.
DESIGN: Ocular evaluations over 6 to 8 months before and after intervention in Cuba.
SETTING: Evaluations performed at a US clinical research facility.
PATIENTS: Ten adult patients aged 25 to 67 years with retinitis pigmentosa.
MAIN OUTCOME MEASURES: Visual acuity, visual field area, and electroretinogram (ERG) amplitude.
RESULTS: No significant change in visual acuity or visual field area was observed on average between preintervention and postintervention values over a 6- to 8-month interval. Mean 30-Hz cone ERG amplitude declined by 15.5% between preintervention and postintervention values (P = .006). When data on change in visual field area from 1 statistically significant outlier were excluded from the analysis, a significant decline of 12.9% in mean visual field area was observed (P = .025).
CONCLUSIONS: These data support the conclusion that the intervention offered in Cuba provides no benefit to patients with retinitis pigmentosa as measured by visual acuity, visual field area, and ERG. The magnitudes of the mean declines observed in ERG amplitude and visual field area over a 6- to 8-month interval, relative to those reported in previous studies, raise the possibility that this intervention may worsen the course of the disease.
Ozone Therapy in Patients with Retinitis Pigmentosa
Mirtha Copelloa, Frank Eguíab, Silvia Menéndezc & Niusdalys Menéndeza OZONE SCIENCE & ENGINEERING,25(3), 223-232. pages 223-232
Abstract
The aim of this study is to determine the efficacy of ozone therapy in patients with Retinitis Pigmentosa (RP). A controlled, randomized, double blind clinical trial involving 68 patients was performed. Patients were divided into 2 groups: ozone, patients treated with ozone by rectal administration (dose=10 mg), during 15 sessions; control, as ozone group, but using oxygen. The main outcome variable was the visual field area (VFA). Results demonstrated a significant improvement (SI) in 88.2% of patients treated with ozone in comparison with 23.5% achieved in the control group. In the ozone group, VFA tend to stabilize beyond a mean time of 6.83 months with a loose in SI afterward. A temporal positive effect of ozone therapy. Over the natural course of RP, was found.
Controlled Clinical Trial on the Use of Ozonated Blood as a Treatment for Retinitis Pigmentosa.
N. Moreno, O Pelaez, T. Aleman, C. Barcelo. International Reference Center of Retinitis Pigmentosa “Camilo Cienfuegos”, Cuba
The aim of this study is to determine the effects of ozone as a therapy for patient with retinitis pigmentosa. A double blind, placebo controlled study was designed, in which 123 patients, ages 15 to 65 years, and suffering from typical forms of retinitis pigmentosa participated Sixty two patients were randomly assigned to the treatment group and 61 to the control group.
A complete general and ophthalmologic examination was performed after signing an informed consent Snellen visual acuity was measured (less than 2.5 lines considered non significant changes) and Goldmann kinetic visual field with 31.5 asb. background and V-4e target. Visual field areas were digitized and measured with a computer program (VISUAL). Ozonated blood was administered to patients in the treatment group, in a daily fashion for 15 days, while patients in the control group received only blood. Participants were blind with regards to this, throughout the duration of the study. Visual acuity remained the same, 6 months after the treatment, in 74.2% of the patients, improve in 21% and worsen in 4.8 %. Visual field area remained the same, 6 months after the treatment, in 41.9% of the patients, improve in 46.7% and worsen in 11.35%.
Significant differences were observed between groups, with lower frequencies of improvements in controls are larger proportions of worsening. Improvements were not observed in 91.9% of patients one year after the treatment. This tendency was first noted after 6 months from the treatment. Ozone treatment is proved of benefit in patients with RP, although its action is temporary .Repeated administration after 6 months is recommendable to maintain its action.
Effects of Ozone in Patients with Retinitis Pigmentosa.
Y. Mapolon, M. Palma, E. Resio, M. Rodríguez, E. Dyce, C. Harrys, J. C. Pina. Retinitis Pigmentosa Center, Camagüey, Cuba. Dr. Eduardo Agramonte Pina Children Hospital, Camagüey, Cuba.
The effects of ozone in 50 patients with retinitis pigmentosa was evaluated in the Retinitis Pigmentosa Center, Camagüey, Cuba. The patients suffer of photophobia abnormal intolerance to visual perception of light), photopsia (flashes of light) and reduce vision on dark places.
Considering that the patients with remission of two symptoms of the disease after the ozone treatment is considered as signs improvement, in this case all of them after the 7th and 8th ozone application didn’t present photophobia and phototopsia, increasing also the quality of their vision (by means of improvement in the visual acuity and visual field). In spite of, after 6 month the appearance of signs and symptoms in all the patients took place. It is considered that the results are important and very useful as alternative in patients that not admit the surgery, repeating it twice a year.
Ozone Therapy in Different Opthalmologic Diseases.
E. C. Díaz, L.Borrego, S. Menéndez, L. R. Borrego, R. A. Borrego. V.I.Lenin; Provincial Hospital, Holguín, Cuba. Provincial Center of Retinosis Pigmentaria, Holguín, Cuba.Ozone Research Center, Cuba. Faculty of Medicine, Holguín , Cuba.
Since 1992, we have been using ozone therapy in our province, like a new therapeutic option in the treatment of Retinitis Pigmentosa. Taking into account the different medical properties of ozone: Improvement of the oxygen transportation to tissue, activation of the enzymes participating in peroxide or oxygen radical scavenging, immunological and metabolic modulator, as well as to be a therapy ease to apply, we decided to use ozone therapy in others ophthalmologic diseases.
180 patients were treated with daily rectal ozone therapy, during 20 days, representing
· 80 with Retinitis Pigmentosa (no systemic, stage I and II),
· 45 with progressive myopia,
· 25 with chronic glaucoma of open angle (tonometrically compensated),
· 20 with optic atrophy (less than one year of evolution and not hereditary),
· 10 with diabetic retinopathy (no proliferative).
Healing criteria was according to each disease. Clinical evaluation was made each 3 months, up to 1 year. In patients with:
Retinitis Pigmentosa, 75% improved their visual acuity (post-treatment and 6 months later).
After 1 year, 23% of improvement still remained. According to visual field, 76% of patients improved it after treatment and up to 9 months, but after 1 year, 16% lost their improvement.
Progressive myopia, the visual acuity increased in 78% (post-treatment and 9 months later) and remained 58% after 1 year.
Glaucoma, 65% increased the visual acuity (post-treatment and 9 months later), maintaining 53% of improvement after 1 year. Visual field increased in 76% of patients, post-treatment and after 1 year.
Diabetic retinopathy, 60% improved their visual acuity (post-treatment), diminishing to 40%, 6 months later and 20%, after 1 year.
Optic atrophy, 45% of improvement in visual field was achieved (post-treatment) maintaining its figure after 1 year.
It is concluded that ozone therapy offers very good results in this opthalmologic diseases, without side effects, being necessary to repeat the treatment in dependence of the disease and the patient evolutions.
Application of Ozone in Patients with Keratitis.
J.C. Pina, Y. Mapolon, M. Palma, E. Recio, C. Harrys, M. Rodríguez. Dr. Eduardo Agramonte Pina Children Hospital, Camagüey, Cuba. Retinitis Pigmentosa Center, Camagüey, Cuba.
Since February 1996, 120 patients with keratitis were treated in the Retinitis Pigmentosa Center of Camagüey, Cuba. 60 patients (sample) were treated with ozone and 60 patients (control group) with conventional treatment (yodoxuridine, leuferon, homatropine, vitamin therapy). 35 males and 45 females, between 15 and 35 years old presenting photophobia and feeling of strange bodies in their eyes were evaluated. At the present, this disease has a long course and the conventional treatment is very expensive, mostly because of the medications it requires. The ozone treatment was rectal application and subconjunctival way, combining both. After the first week, all the patients treated with ozone started to show signs of improvement and they were totally cured after 3 weeks, while in the control group, the evolution had a long course, more than 6 months. We concluded that ozone therapy brings very positive results in the treatment of this disease.
Rehabilitation of Patients with Primary Glaucoma of Open Angle Under Sanatorial Regimen.
L. Ferrer, I. Fernández, M. Ibañez, A. Rodríguez, F. Varela, D. Santos, B. Lazo, R. Marin, I. Jiménez, J.R. Amaro. Carlos J. Finlay Military Hospital, Cuba. Escambray Kurthotel Topes de Collantes, Cuba.Octavio de la Concepci—n y de la Pedraja Hospital, Cuba.
Primary glaucoma of open angle is a disease that can invalidate man in different stages of his physical labor and intellectual life. Several studies in various countries, placed it among the world three primary causes of visual blindness invalidity. Studies carried out by Health World Organization in 1992 reported it as the second cause of blindness in Latin America and the first in the Caribbean area. It is a kind of multifactorial disease for which does not exist a unique solution. Therefore, this element has to be taken into account at the time of a medical therapeutic. Ocular hemodynamic changes are an important factor which play a fundamental role in the raise and development of a glaucoma process. The nervous system participation is another aspect to be considered in this disease. During the first decades of this century a link between the ocular tone and vegetative nervous system has been established. Beside this, rehabilitation treatment with relaxation techniques under sanatorial regimen had been introduced and thereby various factors had contributed to improve its treatment.
131 patients (251 eyes) with diagnosis of open primary angle glaucoma with ages between 20 to 79 years old, remained during 21 days in Escambray Kurthotel “Topes de Collantes”, between January 1995 and October 1996. Visual function and ocular hydrodynamic had been studied in those patients, which were treated with ozone or ozone with magnetic field. Two subgroups had been created: one of then received local medical treatment with Timolol and the other without local treatment. Improvement in the visual functions as well as in ocular hydrodynamic were achieved. Mechanism of action is also reported.
Ozone Therapy in Patients Suffering from Optic Nerve Dysfunction.
R. Santiesteban, S. Menéndez, M. Francisco, S. Luis. Institute of Neurology and Neurosurgery, Cuba. Ozone Research Center, Cuba.
One of the most frequent causes of blindness around the world is optic nerve dysfunction (OND), or optic atrophy, which can be the result of different disturbances affecting the visual pathway like ischemic, toxic, metabolic and hereditary-degenerative phenomena, injuries or compression, among others, producing this type of irreversible sequel. The main object of this study is to evaluate the feasibility to improve the visual function of a group of patients, with different degrees of OND with reduced possibilities of vision improvement, by means of ozone therapy. To 60 patients, suffering from OND of different etiologies and time of evolution, were applied the mixture ozone/oxygen endovenously by autohemotherapy, during 15 sessions. Ozone concentration and doses are used according to the biochemical status of each patient. An ophthalmological examination and a set of tests conformed by visual acuity (VA), visual field by Goldmann Perimetry (VF), visual evoked potentials (VEP), Pelli Robson Contrast Sensitivity Test (PRCST) was applied to patients before and after ozone therapy treatment. PRCST and VF were the parameters mostly improved in patients with 86% and 83% respectively, followed by VA (55%) and VEP (37%). Good results were achieved in all etiologies studied, except Leber optic atrophy, where no improvement was observed, neither objective nor subjective.
Ozone Therapy in Patients with Diabetic Retinopathy.
Hernández R., Ramírez T., Guerrero M., Betancourt J., Rubio L. and Izquierdo N. (Cuba)
The aim of this paper is to evaluate the efficacy of ozone therapy in the treatment of Non-Proliferative Diabetic Retinopathy (NPDR), an ocular complication of diabetes. Twenty five patients were examined through clinic biometry, in order to confirm the absence of other complications of diabetes. The inclusion criteria were: patients between 40 and 60 years old, males and females who suffered loss of visual acuity produced by retinal changes due to diabetic retinopathy. Patients were treated with ozone, by rectal insufflation, at concentration between 35-40 mg/L and 200 mL, one daily, for 15 sessions. This cycle of treatment was repeated every six months, up to 2 years. An ophthalmologic evaluation (visual acuity, direct and indirect ophthalmoscopy, retinophoto and fluorescein angiography) of the patients was performed at the beginning and at the end of every cycle of ozone therapy.
In 72% of the patients a visual improvement was achieved, with a slow disappearance of microaneurysm, intraretinal hemorrhages, hard exudates, microhemorrhages and retinal edema. From the second cycle on, this improvement was maintained in direct relation with a clinic biometry within the normal parameters (cholesterol, glycemia and creatinine), mainly on patients who suffered from Diabetes type II. No visual improvement was observed in the rest of the patients (28%) during 2 years of follow-up. An appropriate metabolic control of patients who suffer from mild NPDR was achieved, allowing a favorable recovering of their vision, as well as an improvement in their quality of life. On the basis of the stimulant effect of medical ozone in the processes of oxygen metabolization and blood circulation we can recommend this therapy for the treatment of patients suffering of Non-Proliferative Diabetic Retinopathy.